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验证数据展示

  • WB analysis using 26501-1-AP

    WB analysis using 26501-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26501-1-AP (AMBN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 26501-1-AP

    IF Staining of HEK-293 using 26501-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using AMBN antibody (26501-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

AMBN Polyclonal antibody

AMBN Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26501-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Ameloblastin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26501-1-AP targets AMBN in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 AMBN fusion protein Ag24129 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ameloblastin (enamel matrix protein)
别名 Ameloblastin
计算分子量 447 aa, 48 kDa
观测分子量48-55 kDa
GenBank蛋白编号BC106931
基因名称 AMBN
Gene ID (NCBI) 258
RRIDAB_3669542
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NP70
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMBN(Ameloblastin), the gene encodes the nonamelogenin enamel matrix protein ameloblastin. The encoded protein may be important in enamel matrix formation and mineralization. Mutations in this gene may be associated with dentinogenesis imperfect and autosomal dominant amylogenesis imperfect. It is expected to be located in the Vesicles, in addition localized to the Nucleoplasm, which is enriched in brain, and located at the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel. The molecular weight of AMBN is 48 kDa. It also has phosphorylation modification.

实验方案

Product Specific Protocols
WB protocol for AMBN antibody 26501-1-APDownload protocol
IF protocol for AMBN antibody 26501-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols