验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30963-1-AP targets AGTR2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag33887 Product name: Recombinant human AGTR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-45 aa of NM_000686 Sequence: MKGNSTLATTSKNITSGLHFGLVNISGNNESTLNCSQKPSDKHLD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | angiotensin II receptor, type 2 |
| 别名 | AGTR 2, Angiotensin II type 2 receptor, AT2, ATGR2, Type 2 angiotensin II receptor |
| 计算分子量 | 41 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | NM_000686 |
| 基因名称 | AGTR2 |
| Gene ID (NCBI) | 186 |
| RRID | AB_3669797 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P50052 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AGTR2 (angiotensin II type 2 receptor) is part of the renin-angiotensin signaling (RAS) pathway that has been widely studied for its role in blood pressure regulation and renal and cardiovascular health (PMID: 29937318). AGTR2 mediates the effects of angiotensin II on cellular differentiation and growth (PMID: 30455538).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for AGTR2 antibody 30963-1-AP | Download protocol |
| WB protocol for AGTR2 antibody 30963-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
