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ADRB3 Polyclonal antibody

ADRB3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 30713-1-AP

Print datasheet

Synonyms

ADRB3, ADRB3R, adrenergic, beta 3 , receptor, B3AR, Beta 3 adrenergic receptor, Beta 3 adrenoceptor, Beta 3 adrenoreceptor, BETA3AR, FLJ99960

验证数据展示

  • WB analysis using 30713-1-AP

    WB analysis using 30713-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30713-1-AP (ADRB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30713-1-AP targets ADRB3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen ADRB3 fusion protein Ag31466 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adrenergic, beta-3-, receptor
Synonyms ADRB3, ADRB3R, adrenergic, beta 3 , receptor, B3AR, Beta 3 adrenergic receptor, Beta 3 adrenoceptor, Beta 3 adrenoreceptor, BETA3AR, FLJ99960
Calculated Molecular Weight 44 kDa
Observed Molecular Weight44-50 kDa
GenBank Accession NumberNM_000025
Gene Symbol ADRB3
Gene ID (NCBI) 155
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-3 adrenergic receptor(ADRB3), a member of the G-protein-coupled receptor family, is expressed mainly in adipose tissue and is thought to contribute to lipolysis and thermogenesis. In addition, the overexpression of ADRB3 has been found in several cancer types, including breast cancer, gallbladder cancer , and colorectal cancer. More recently, the involvement of ADRB3 in the metabolic reprogramming of melanoma, the promotion of immune tolerance, and the regulation of cancer differentiation have been described. (PMID: 32514619)

实验方案

Product Specific Protocols
WB protocol for ADRB3 antibody 30713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols