Beta Actin Monoclonal antibody
beta actin Monoclonal Antibody for ELISA
Host / Isotype
Mouse / IgM
Reactivity
human and More (3)
Applications
ELISA and More (3)
Conjugate
Unconjugated
CloneNo.
7A9H3
验证数据展示
产品信息
60008-2-Ig targets beta actin in WB, IP, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | ELISA |
| Cited Applications | WB, IP, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, bovine |
| Immunogen | beta actin fusion protein Ag0297 种属同源性预测 |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | actin, beta |
| Synonyms | |
| Calculated Molecular Weight | 375 aa, 42 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC002409 |
| Gene Symbol | ACTB |
| Gene ID (NCBI) | 60 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Caprylic Acid/Ammonium Sulfate Precipitation |
| UNIPROT ID | P60709 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins.
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression. | ||
Elife Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors. | ||
Bioeng Transl Med Neonatal microglia and proteinase inhibitors-treated adult microglia improve traumatic brain injury in rats by resolving the neuroinflammation. | ||
PLoS Genet Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing. |
