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验证数据展示

  • WB analysis using 32036-1-AP

    WB analysis using 32036-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32036-1-AP (ABHD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ABHD4 Polyclonal antibody

ABHD4 Polyclonal Antibody for WB, ELISA
Cat No. 32036-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

Alpha/beta-hydrolase 4, Alpha/beta hydrolase domain-containing protein 4, Abhydrolase domain-containing protein 4, Abhydrolase Domain Containing 4, N-Acyl Phospholipase B, Abhydrolase Domain Containing 4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, LNCap cells, SiHa cells, mouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32036-1-AP targets ABHD4 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag36373

Product name: Recombinant human ABHD4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 250-342 aa of BC024779

Sequence: PSGETAFKAMMESFGWARRPMLERIHLIRKDVPITMIYGSDTWIDTSTGKKVKMQRPDSYVRDMEIKGASHHVYADQPHIFNAVVEEICDSVD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 abhydrolase domain containing 4
别名 Alpha/beta-hydrolase 4, Alpha/beta hydrolase domain-containing protein 4, Abhydrolase domain-containing protein 4, Abhydrolase Domain Containing 4, N-Acyl Phospholipase B, Abhydrolase Domain Containing 4
计算分子量 342 aa, 39 kDa
观测分子量39 kDa
GenBank蛋白编号BC024779
基因名称 ABHD4
Gene ID (NCBI) 63874
RRIDAB_3670176
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8TB40
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The alpha/beta hydrolase structural domain-containing protein 4 (ABHD4) gene is a major regulator of mammalian phospholipid metabolism with hydrolase and lysophospholipase activities. ABHD4 is involved in anoikis resistance and endogenous cannabinoid biosynthesis, and ABHD4-mediated developmental anoikis specifically protects embryonic brains from the consequences of sporadic delamination errors and teratogenic damage.

实验方案

Product Specific Protocols
WB protocol for ABHD4 antibody 32036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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