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验证数据展示

  • WB analysis of HeLa using 22558-1-AP

    WB analysis of HeLa using 22558-1-AP

    WB result of ABHD10 antibody (22558-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABHD10 transfected HeLa cells.

  • WB analysis using 22558-1-AP

    WB analysis using 22558-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 22558-1-AP (ABHD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

ABHD10 Polyclonal antibody

ABHD10 Polyclonal Antibody for WB, ELISA
Cat No. 22558-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inY79 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22558-1-AP targets ABHD10 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 ABHD10 fusion protein Ag18413 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 abhydrolase domain containing 10
别名
计算分子量 306 aa, 34 kDa
观测分子量28 kDa
GenBank蛋白编号BC014516
基因名称 ABHD10
Gene ID (NCBI) 55347
RRIDAB_3669401
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NUJ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABHD10 is a mitochondrial protein that is a member of the APT family. It has 3 isoforms and regulates the S-palmitoylation status of the nucleophilic active site residue of peroxiredoxin-5 (PRDX5) (PMID: 31740833).

实验方案

Product Specific Protocols
WB protocol for ABHD10 antibody 22558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols