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验证数据展示

  • WB analysis of mouse heart using 13815-1-AP (same clone as 13815-1-PBS)

    WB analysis of mouse heart using 13815-1-AP (same clone as 13815-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13815-1-AP (AACS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 13815-1-AP (same clone as 13815-1-PBS)

    IF Staining of MCF-7 using 13815-1-AP (same clone as 13815-1-PBS)

    Immunofluorescent analysis of MCF-7 cells using 13815-1-AP (AACS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 13815-1-AP (same clone as 13815-1-PBS)

    IHC staining of human ovary cancer using 13815-1-AP (same clone as 13815-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13815-1-AP (AACS antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

AACS Polyclonal antibody, PBS Only

AACS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13815-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Protein sur 5 homolog, EC:6.2.1.16, Acyl-CoA synthetase family member 1, ACSF1, ACSF 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

13815-1-PBS targets AACS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AACS fusion protein Ag4899 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acetoacetyl-CoA synthetase
别名 Protein sur 5 homolog, EC:6.2.1.16, Acyl-CoA synthetase family member 1, ACSF1, ACSF 1
计算分子量 672 aa, 75 kDa
观测分子量 67 kDa, 30 kDa
GenBank蛋白编号BC040490
基因名称 AACS
Gene ID (NCBI) 65985
RRIDAB_2877977
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86V21
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AACS(acetoacetyl-CoA synthetase) is also named as ACSF1 and belonogs to the ATP-dependent AMP-binding enzyme family. In mammals, AACS is present in the cytosolic fractions of various tissues, especially lipogenic ones, and acetoacetate is known to be effectively incorporated into cholesterol and fatty acids. These results indicate that AACS may supply acetyl units to cytosolic compartment for cholesterol and/or fatty acid biosynthesis. It has 3 isoforms produced by alternative splicing.