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验证数据展示

  • WB analysis using 27674-1-AP

    WB analysis using 27674-1-AP

    Human saliva was subjected to SDS PAGE followed by western blot with 27674-1-AP (A2ML1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

A2ML1 Polyclonal antibody

A2ML1 Polyclonal Antibody for WB, ELISA
Cat No. 27674-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

A2ML1, alpha 2 macroglobulin like 1, CPAMD9, Homo sapiens (Human)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman saliva

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27674-1-AP targets A2ML1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 A2ML1 fusion protein Ag25818 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alpha-2-macroglobulin-like 1
别名 A2ML1, alpha 2 macroglobulin like 1, CPAMD9, Homo sapiens (Human)
计算分子量 1454 aa, 161 kDa
观测分子量161 kDa
GenBank蛋白编号BC112131
基因名称 A2ML1
Gene ID (NCBI) 144568
RRIDAB_3085984
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA8K2U0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

A2ML1 is a member of the alpha-macroglobulin superfamily. It is thought to be an N-glycosylated monomeric protein that acts as an inhibitor of several proteases. It has been shown to form covalent interactions with proteases, and has been reported as the p170 antigen recognized by autoantibodies in the autoimmune disease paraneoplastic pemphigus.

实验方案

Product Specific Protocols
WB protocol for A2ML1 antibody 27674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols