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3-Methylcytosine (3-mC) antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Not Species Specific

Applications

IF, Dot Blot, ICC

Cat No : 61111,61112 61111

Synonyms

DNA methylation, S-adenosyl methionine, SAM, 3-methylcytosine, 3-meC, 3 methylcytosine, 3-methycytidine, 3 methylcytosine, 3-methylC, base excision repair, BER, dealkylation, AlkB, methylcytosine, sample

验证数据展示

  • 3-Methylcytosine (3-mC, 3-methylcytidine) antibody (pAb) tested by DNA dot blot BSA conjugated nucleosides (starting at 10ng as indicated) were spotted onto PVDF membrane and blotted with 3-methylcytidine antibody at a dilution of 1:10,000. Lane 1: 3-methylcytidine. Lane 2: Cytidine. Lane 3: 5-methylcytidine. Lane 4: 5-hydroxymethylcytidine.

    3-Methylcytosine (3-mC, 3-methylcytidine) antibody (pAb) tested by DNA dot blot BSA conjugated nucleosides (starting at 10ng as indicated) were spotted onto PVDF membrane and blotted with 3-methylcytidine antibody at a dilution of 1:10,000. Lane 1: 3-methylcytidine. Lane 2: Cytidine. Lane 3: 5-methylcytidine. Lane 4: 5-hydroxymethylcytidine.

产品信息

Tested Applications IF, Dot Blot, ICC

Applications Validated by Active Motif: DB: 0.1 - 1 ug/ml dilution
Tested Reactivity Human, Mouse, Not Species Specific
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
ModificationMethylated
Immunogen This 3-methylcytosine antibody was raised against 3-methylcytidine conjugated to KLH and recognizes 3-methylcytosine.
Full Name 3-Methylcytosine (3-mC) antibody (pAb)
Synonyms DNA methylation, S-adenosyl methionine, SAM, 3-methylcytosine, 3-meC, 3 methylcytosine, 3-methycytidine, 3 methylcytosine, 3-methylC, base excision repair, BER, dealkylation, AlkB, methylcytosine, sample
Molecular weight
GenBank accession numberN/A
RRIDAB_2793510
Purification Method Protein A Chromatography
Buffer Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

Methylation of DNA can occur non-enzymatically at the nitrogen-three of the cytosine base through spontaneous exposure to endogenous S-adenosyl methionine (SAM). The resulting 3-methylcytosine (3-mC) is mutagenic and must be repaired, which occurs in humans through the base excision repair (BER) or dealkylation via human homologues of the E. coli AlkB protein. 3-methylcytosine is present in human cell lines and increased levels of 3-mC impair proliferation.