Recombinant Human AGRN protein (rFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human AGRN protein Ala1260-Pro2045 (Accession# O00468-6) with a rabbit IgG Fc tag at the N-terminus. |
| 基因ID | 375790 |
| 蛋白编号 | O00468-6 |
| 预测分子量 | 109.5 kDa |
| SDS-PAGE | 100-140 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Agrin, a large heparan sulfate proteoglycan, is expressed in neuronal, as well as nonneuronal tissues. Nerve-derived agrin has been demonstrated to play an essential role in development and maintenance of the neuromuscular junction. The agrin gene encodes a protein of more than 2000 amino acids with predicted molecular weight of 225 kDa but can display as 600 kDa protein due to extensive N-terminal glycosylation. Neurotrypsin cleaves the proteoglycan agrin at two homologous, highly conserved sites, liberating a middle 90-kDa and a C-terminal 22-kDa fragment from the membrane or ECM-bound N-terminal moiety of agrin.
参考文献:
1. Bezakova, Gabriela, and Markus A Ruegg. Nature reviews. Molecular cell biology vol. 4,4 (2003): 295-308. 2. Kummer, Terrance T et al. Current opinion in neurobiology vol. 16,1 (2006): 74-82. 3. Stephan, Alexander et al. FASEB journal : official publication of the Federation of American Societies for Experimental Biology vol. 22,6 (2008): 1861-73.
