Recombinant Human KLK14 protein (rFc Tag)(HPLC verified)
种属
Human
纯度
>90 %, SDS-PAGE
>90 %, SEC-HPLC
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE >90 %, SEC-HPLC |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human KLK14 protein Gln35-Lys267 (Accession# Q9P0G3) with a rabbit IgG Fc tag at the C-terminus. |
| 基因ID | 43847 |
| 蛋白编号 | Q9P0G3 |
| 预测分子量 | 51.5 kDa |
| SDS-PAGE | 13 kDa, 28-32 kDa and 55 kDa, reducing (R) condition |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
KLK14, or Kallikrein-14, is a protein-coding gene that belongs to the kallikrein family of secreted serine proteases. Activation of KLK14 is mediated by KLK5, and once activated, KLK14 further amplifies the activity of KLK proteases through a positive feedback loop by cleaving pro-KLK5, which is a central player in the KLK cascade. One of the most notable substrates of KLK14 is PAR2 (Protease-Activated Receptor 2). KLK14 has been implicated in various physiological functions and is suggested to be involved in carcinogenesis, with potential as a novel biomarker for diseases such as cancer and skin disorders. It is associated with diseases like Netherton Syndrome and Ovarian Cancer. Moreover, KLK14 may play a role in the activation of membrane-type matrix metalloproteinases (MT-MMPs), which are involved in the proteolytic processing of components of the extracellular matrix, modulating the pericellular environment in physiology and pathologies.
参考文献:
1. Katherine Falkowski . et al. (2020). Int J Mol Sci. 21(12):4383. 2. Hyesook Yoon. et al. (2007). J Biol Chem.282(44):31852-31864. 3. Hyun-Jeong Ra. et al. (2007). Matrix Biol. 26(8):587-596.
