验证数据展示
经过测试的应用
| Positive FC detected in | Transfected CHO |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98582-1-RR targets TNFSF12 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg2870 Product name: Recombinant Human TNFSF12 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 93-249 aa of NM_003809.2 Sequence: RSAPKGRKTRARRAIAAHYEVHPRPGQDGAQAGVDGTVSGWEEARINSSSPLRYNRQIGEFIVTRAGLYYLYCQVHFDEGKAVYLKLDLLVDGVLALRCLEEFSATAASSLGPQLRLCQVSGLLALRPGSSLRIRTLPWAHLKAAPFLTYFGLFQVH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor (ligand) superfamily, member 12 |
| 别名 | CD255, DR3LG, hCG_1991317, HCG1991317, isoform CRA_b TWEAK Tumor necrosis factor, Ligand |
| 计算分子量 | 27 kDa |
| GenBank蛋白编号 | NM_003809.2 |
| 基因名称 | TNFSF12 |
| Gene ID (NCBI) | 8742 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O43508-1 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
TNFSF12, also known as TWEAK (TNF-like weak inducer of apoptosis), is a member of the tumor necrosis factor ligand superfamily. It functions as a cytokine by binding to its primary receptor, Fn14 (TNFRSF12A), and plays diverse roles in immune regulation, angiogenesis, tissue repair, and inflammation (PMID: 39905000). TNFSF12 is upregulated in multiple solid tumors and promotes cancer cell proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT) (PMID: 28639899).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for TNFSF12 antibody 98582-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
