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验证数据展示

  • FC experiment of MG-63 using 98405-2-RR

    FC experiment of MG-63 using 98405-2-RR

    1x10^6 MG-63 cells were surface stained with 0.25 ug Anti-Human PDGFR beta/CD140b Rabbit RecAb (98405-2-RR, Clone: 242711A9) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • FC experiment of Transfected HEK-293T using 98405-2-RR

    FC experiment of Transfected HEK-293T using 98405-2-RR

    1x10^6 HEK-293T cells or PDGFR beta/CD140b transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human PDGFR beta/CD140b Rabbit RecAb (98405-2-RR, Clone: 242711A9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • FC experiment of Transfected HEK-293T using 98405-2-RR

    FC experiment of Transfected HEK-293T using 98405-2-RR

    1x10^6 HEK-293T cells (left) or PDGFR beta/CD140b transfected HEK-293T cells(right) were surface stained with 0.25 ug Anti-Human PDGFRB Rabbit RecAb (98405-2-RR, Clone: 242711A9) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

Anti-Human PDGFR beta/CD140b Rabbit Recombinant Antibody

PDGFR beta/CD140b Recombinant Antibody for FC
Cat No. 98405-2-RR

产品说明书

CloneNo. 242711A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inMG-63 cells, Transfected HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy PDGFRB Ready-To-Use IHC Kit. PDGFRB primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98405-2-RR targets PDGFR beta/CD140b in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 PDGFR beta/CD140b fusion protein Eg3715 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 platelet-derived growth factor receptor, beta polypeptide
别名 PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B
计算分子量 1106 aa, 124 kDa
GenBank蛋白编号BC032224
基因名称 PDGFR beta
Gene ID (NCBI) 5159
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09619
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

PDGFRs are receptor tyrosine kinases with an extracellular ligand-binding domain and a cytoplasmic enzymatic domain. The signaling activity of PDGFRs is tightly regulated by an autoinhibitory allosteric conformation, and requires ligand-induced receptor dimerization at the cell surface to alleviate autoinhibition and achieve physiological activity (PMID: 21664579). PDGFR beta (platelet-derived growth factor receptor- β)/CD140b is a constitutive marker expressed by ASCs in the angiogenic behavior of human retinal endothelial cells (HREs). CD140b (PDGFRβ) plays a crucial role in controlling the intrinsic proliferation (PMID: 30976657)

实验方案

Product Specific Protocols
FC protocol for PDGFR beta/CD140b antibody 98405-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols