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验证数据展示

  • Dot Blot experiment of / using 87006-1-RR

    Dot Blot experiment of / using 87006-1-RR

    Dot blot analysis was used to confirm the specificity of 86990-2-RR Di-Methyl-Histone H4 (Lys20) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • WB analysis using 87006-1-RR

    WB analysis using 87006-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87006-1-RR (Di-Methyl-Histone H4 (Lys20) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 87006-1-RR

    IHC staining of mouse colon using 87006-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87006-1-RR (Di-Methyl-Histone H4 (Lys20) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 87006-1-RR

    IHC staining of mouse colon using 87006-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87006-1-RR (Di-Methyl-Histone H4 (Lys20) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 87006-1-RR

    IF Staining of HeLa using 87006-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Di-Methyl-Histone H4 (Lys20) antibody (87006-1-RR, Clone: 251934G9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Di-Methyl-Histone H4 (Lys20) Recombinant monoclonal antibody

Di-Methyl-Histone H4 (Lys20) Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, ELISA
Cat No. 87006-1-RR

产品说明书

CloneNo. 251934G9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Dot Blot, ELISA

别名

Di Methyl Histone H4, Di-Methyl Histone H4 (Lys20), Di-Methyl Histone H4 Lys20, Di-Methyl Histone-H4 Lys20, Di-Methyl-Histone H4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, NIH/3T3 cells, HSC-T6 cells, mouse liver tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected in/ /

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87006-1-RR targets Di-Methyl-Histone H4 (Lys20) in WB, IHC, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Dot Blot, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H4a
别名 Di Methyl Histone H4, Di-Methyl Histone H4 (Lys20), Di-Methyl Histone H4 Lys20, Di-Methyl Histone-H4 Lys20, Di-Methyl-Histone H4
观测分子量15 kDa
GenBank蛋白编号BC069654
基因名称 HIST1H4A
Gene ID (NCBI) 8359
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62805
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Di-Methyl-Histone H4 (Lys20) antibody 87006-1-RRDownload protocol
IHC protocol for Di-Methyl-Histone H4 (Lys20) antibody 87006-1-RRDownload protocol
WB protocol for Di-Methyl-Histone H4 (Lys20) antibody 87006-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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