Dot blot analysis was used to confirm the specificity of 86990-2-RR Di-Methyl-Histone H4 (Lys20) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 87006-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 87006-1-RR (Di-Methyl-Histone H4 (Lys20) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87006-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87006-1-RR (Di-Methyl-Histone H4 (Lys20) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87006-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87006-1-RR (Di-Methyl-Histone H4 (Lys20) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87006-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Di-Methyl-Histone H4 (Lys20) antibody (87006-1-RR, Clone: 251934G9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87006-1-PBS in a different storage buffer formulation.