验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, LNCaP cells, mouse liver tissue, rat liver tissue, mouse brain tissue, mouse pancreas tissue |
| Positive IP detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86747-1-RR targets GUF1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11579 Product name: Recombinant human GUF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 318-669 aa of BC036768 Sequence: VGYLIAGMKDVTEAQIGDTLCLHKQPVEPLPGFKSAKPMVFAGMYPLDQSEYNNLKSAIEKLTLNDSSVTVHRDSSLALGAGWRLGFLGLLHMEVFNQRLEQEYNASVILTTPTVPYKAVLSSSKLIKEHREKEITIINPAQFPDKSKVTEYLEPVVLGTIITPDEYTGKIMMLCEARRAVQKNMIFIDQNRVMLKYLFPLNEIVVDFYDSLKSLSSGYASFDYEDAGYQTAELVKMDILLNGNTVEELVTVVHKDKAHSIGKAICERLKDSLPRQLFEIAIQAAIGSKIIARETVKAYRKNVLAKCYGGDITRKMKLLKRQAEGKKKLRKIGNVEVPKDAFIKVLKTQSSK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | GUF1 GTPase homolog (S. cerevisiae) |
| 别名 | EC:3.6.5.-, EF-4, Elongation factor 4 homolog, GTPase GUF1, Ribosomal back-translocase |
| 计算分子量 | 669 aa, 74 kDa |
| 观测分子量 | 68-70 kDa |
| GenBank蛋白编号 | BC036768 |
| 基因名称 | GUF1 |
| Gene ID (NCBI) | 60558 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8N442 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for GUF1 antibody 86747-1-RR | Download protocol |
| WB protocol for GUF1 antibody 86747-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
