• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86238-5-RR

    WB analysis using 86238-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86238-5-RR (Pan-Keratin (Type I) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 86238-5-RR

    IHC staining of human tonsillitis using 86238-5-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 86238-5-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 86238-5-RR

    IHC staining of human tonsillitis using 86238-5-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 86238-5-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsil using 86238-5-RR

    IF Staining of human tonsil using 86238-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsil tissue using Pan-Keratin (Type I) antibody (86238-5-RR, Clone: 251001E5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsil using 86238-5-RR

    IF Staining of human tonsil using 86238-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsil tissue using Pan-Keratin (Type I) antibody (86238-5-RR, Clone: 251001E5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Pan-Keratin (Type I) Recombinant monoclonal antibody

Pan-Keratin (Type I) Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 86238-5-RR

产品说明书

CloneNo. 251001E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

Pan Keratin, keratin, pan-CK, Pan-CK (Type I), Pan-Keratin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHaCaT cells, A431 cells, HT-29 cells, MCF-7 cells
Positive IHC detected inhuman tonsillitis tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsil tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86238-5-RR targets Pan-Keratin (Type I) in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Pan-Keratin (Type I)
别名 Pan Keratin, keratin, pan-CK, Pan-CK (Type I), Pan-Keratin
观测分子量44-50 kDa
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15323
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type I keratins (or Type I cytokeratins) are acidic, low molecular weight (40-64 kDa) proteins that form Type I intermediate filaments (IFs) in the cytoplasmic cytoskeleton of all mammalian epithelial cells. They are encoded on chromosome 17q and include keratins K9-K10, K12-K28, and K31-K40.

实验方案

Product Specific Protocols
IF protocol for Pan-Keratin (Type I) antibody 86238-5-RRDownload protocol
IHC protocol for Pan-Keratin (Type I) antibody 86238-5-RRDownload protocol
WB protocol for Pan-Keratin (Type I) antibody 86238-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...