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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of rat plasma using 84782-5-RR (same clone as 84782-5-PBS)

    WB analysis of rat plasma using 84782-5-RR (same clone as 84782-5-PBS)

    rat plasma were subjected to SDS PAGE followed by western blot with 84782-5-RR (IGF-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84782-5-PBS in a different storage buffer formulation.

  • FC experiment of Transfected HEK-293T using 84782-5-RR (same clone as 84782-5-PBS)

    FC experiment of Transfected HEK-293T using 84782-5-RR (same clone as 84782-5-PBS)

    1x10^6 HEK-293T cells or IGF1 Transfected HEK-293T cells were intracellularly stained with 0.25 ug IGF1 Recombinant antibody (84782-5-RR, Clone:241890G6) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84782-5-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 84782-5-RR (same clone as 84782-5-PBS)

    IHC staining of rat liver using 84782-5-RR (same clone as 84782-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84782-5-RR (IGF1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84782-5-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 84782-5-RR (same clone as 84782-5-PBS)

    IHC staining of rat liver using 84782-5-RR (same clone as 84782-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84782-5-RR (IGF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84782-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84782-5-RR

    Affinity and Kinetic Characterization of 84782-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84782-5-RR against Rat IGF1 were performed. The affinity constant is below 1 pM.

Rat IGF1 Recombinant antibody, PBS Only

IGF1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 84782-5-PBS

产品说明书

CloneNo. 241890G6

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

241890G6, Igf-1, IGF-I, Insulin-like growth factor I, Somatomedin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84782-5-PBS targets IGF1 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 rat
免疫原 IGF1 fusion protein Eg1976 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 insulin-like growth factor 1
别名 241890G6, Igf-1, IGF-I, Insulin-like growth factor I, Somatomedin
计算分子量14kd
观测分子量12 kDa
GenBank蛋白编号AAA41386
基因名称 Igf1
Gene ID (NCBI) 24482
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08025
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IGF1, also named as IBP1, MGF, IGF-IA, and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but has a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.