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验证数据展示

  • WB analysis using 83596-4-RR (same clone as 83596-4-PBS)

    WB analysis using 83596-4-RR (same clone as 83596-4-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83596-4-RR (BARHL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83596-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83596-4-RR (same clone as 83596-4-PBS)

    IF Staining of HepG2 using 83596-4-RR (same clone as 83596-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (83596-4-RR, Clone: 240558G8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83596-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83596-4-RR (same clone as 83596-4-PBS)

    IF Staining of HepG2 using 83596-4-RR (same clone as 83596-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (83596-4-RR, Clone: 240558G8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83596-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00582-2

    Cytometric bead array standard curve of MP00582-2

    Cytometric bead array standard curve of MP00582-2, BARHL2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83596-1-PBS. Detection antibody: 83596-4-PBS. Standard: Ag21156. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83596-4-RR

    Affinity and Kinetic Characterization of 83596-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83596-4-RR against Human BARHL2 were performed. The affinity constant is 0.478 nM.

BARHL2 Recombinant antibody, PBS Only (Detector)

BARHL2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83596-4-PBS

产品说明书

CloneNo. 240558G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

BarH-like 2 homeobox protein, BarH like 2 homeobox protein, 240558G8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83596-4-PBS targets BARHL2 as part of a matched antibody pair:

MP00582-2: 83596-1-PBS capture and 83596-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BARHL2 fusion protein Ag21156 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BarH-like homeobox 2
别名 BarH-like 2 homeobox protein, BarH like 2 homeobox protein, 240558G8
计算分子量 387 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC126439
基因名称 BARHL2
Gene ID (NCBI) 343472
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NY43
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BARHL2 also named as BarH like homeobox 2 is 279 amino acid protein, which contains 1 homeobox DNA-binding domain. BARHL2 localizes in nucleus and belongs to BAR homeobox family. BARHL2 as a transcription factor binds optimally to the DNA consensus sequence. Barx2 may differentially control the expression of L1 and other target genes during embryonic development. Barx2 is expressed in several smooth muscle-containing tissues, as well as skeletal muscle, brain, tongue and esophagus.