• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 83481-5-RR

    WB analysis using 83481-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83481-5-RR (RPP30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 83481-5-RR

    WB analysis of HEK-293 using 83481-5-RR

    WB result of RPP30 antibody (83481-5-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPP30 transfected HEK-293 cells.

  • IHC staining of human ovary cancer using 83481-5-RR

    IHC staining of human ovary cancer using 83481-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83481-5-RR (RPP30 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 83481-5-RR

    IHC staining of human ovary cancer using 83481-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83481-5-RR (RPP30 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 83481-5-RR

    IF Staining of U-251 using 83481-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RPP30 antibody (83481-5-RR, Clone: 240468E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of U-251 using 83481-5-RR

    IF Staining of U-251 using 83481-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RPP30 antibody (83481-5-RR, Clone: 240468E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of U-251 using 83481-5-RR

    FC experiment of U-251 using 83481-5-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug RPP30 Recombinant antibody (83481-5-RR, Clone:240468E4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83481-5-RR

    Affinity and Kinetic Characterization of 83481-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83481-5-RR against Human RPP30 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

RPP30 Recombinant monoclonal antibody

RPP30 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83481-5-RR

产品说明书

CloneNo. 240468E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

240468E4, RNase P subunit 2, RNaseP protein p30, RNASEP2, TSG15

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, U-251 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83481-5-RR targets RPP30 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8600

Product name: Recombinant human RPP30 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-268 aa of BC006991

Sequence: MAVFADLDLRAGSDLKALRGLVETAAHLGYSVVAINHIVDFKEKKQEIEKPVAVSELFTTLPIVQGKSRPIKILTRLTIIVSDPSHCNVLRATSSRARLYDVVAVFPKTEKLFHIACTHLDVDLVCITVTEKLPFYFKRPPINVAIDRGLAFELVYSPAIKDSTMRRYTISSALNLMQICKGKNVIISSAAERPLEIRGPYDVANLGLLFGLSESDAKAAVSTNCRAALLHGETRKTAFGIISTVKKPRPSEGDEDCLPASKKAKCEG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribonuclease P/MRP 30kDa subunit
别名 240468E4, RNase P subunit 2, RNaseP protein p30, RNASEP2, TSG15
计算分子量 268 aa, 29 kDa
观测分子量30 kDa
GenBank蛋白编号BC006991
基因名称 RPP30
Gene ID (NCBI) 10556
RRIDAB_3671109
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP78346
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPP30 is a part of multimeric ribonuclease P complex and ribonuclease MRP complex. It is also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.

实验方案

Product Specific Protocols
IF protocol for RPP30 antibody 83481-5-RRDownload protocol
IHC protocol for RPP30 antibody 83481-5-RRDownload protocol
WB protocol for RPP30 antibody 83481-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...