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验证数据展示

  • WB analysis using 82802-10-RR

    WB analysis using 82802-10-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82802-10-RR (CISD2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 82802-10-RR

    IHC staining of mouse kidney using 82802-10-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82802-10-RR (CISD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 82802-10-RR

    IHC staining of mouse kidney using 82802-10-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82802-10-RR (CISD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 82802-10-RR

    IF Staining of HepG2 using 82802-10-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CISD2 antibody (82802-10-RR, Clone: 6H13 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • FC experiment of HepG2 using 82802-10-RR

    FC experiment of HepG2 using 82802-10-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CISD2 Recombinant antibody (82802-10-RR, Clone:6H13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82802-10-RR

    Affinity and Kinetic Characterization of 82802-10-RR

    Biolayer interferometry (BLl) kinetic assays of 82802-10-RR against Human CISD2 were performed. The affinity constant is 3.07 nM.

CISD2 Recombinant monoclonal antibody

CISD2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82802-10-RR

产品说明书

CloneNo. 6H13

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Miner1, ERIS, CDGSH2, CDGSH iron-sulfur domain-containing protein 2, CDGSH iron sulfur domain 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HuH-7 cells, L02 cells, K-562 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82802-10-RR targets CISD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4172

Product name: Recombinant human CISD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 61-135 aa of BC032300

Sequence: PKKKQQKDSLINLKIQKENPKVVNEINIEDLCLTKAAYCRCWRSKTFPACDGSHNKHNELTGDNVGPLILKKKEV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CDGSH iron sulfur domain 2
别名 Miner1, ERIS, CDGSH2, CDGSH iron-sulfur domain-containing protein 2, CDGSH iron sulfur domain 2
计算分子量 135 aa, 15 kDa
观测分子量 15 kDa
GenBank蛋白编号BC032300
基因名称 CISD2
Gene ID (NCBI) 493856
RRIDAB_3670556
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8N5K1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum.

实验方案

Product Specific Protocols
FC protocol for CISD2 antibody 82802-10-RRDownload protocol
IF protocol for CISD2 antibody 82802-10-RRDownload protocol
IHC protocol for CISD2 antibody 82802-10-RRDownload protocol
WB protocol for CISD2 antibody 82802-10-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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