验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A549 cells, U2OS cells, K-562 cells, Daudi cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68060-1-Ig targets GEN1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | GEN1 fusion protein Ag31247 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Gen homolog 1, endonuclease (Drosophila) |
| 别名 | Gen, 1A10D10 |
| 计算分子量 | 103 kDa |
| 观测分子量 | 98-100 kDa |
| GenBank蛋白编号 | NM_182625 |
| 基因名称 | GEN1 |
| Gene ID (NCBI) | 348654 |
| RRID | AB_2918801 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q17RS7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GEN1 is a endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. GEN1 efficiently cleaves both single and double HJs contained within large recombination intermediates.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GEN1 antibody 68060-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |