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CoraLite® Plus 488-conjugated GEN1 Monoclonal antibody

GEN1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A10D10

Cat No : CL488-68060

Print datasheet

Synonyms

DKFZp781F0986, FLJ40869, Gen, GEN1

验证数据展示

  • FC experiment of A431 using CL488-68060

    FC experiment of A431 using CL488-68060

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GEN1 (CL488-68060, Clone:1A10D10) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68060-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68060 targets GEN1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen GEN1 fusion protein Ag31247 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Gen homolog 1, endonuclease (Drosophila)
Synonyms DKFZp781F0986, FLJ40869, Gen, GEN1
Calculated Molecular Weight 103 kDa
Observed Molecular Weight98-100 kDa
GenBank Accession NumberNM_182625
Gene Symbol GEN1
Gene ID (NCBI) 348654
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ17RS7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GEN1 is a endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. GEN1 efficiently cleaves both single and double HJs contained within large recombination intermediates.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 GEN1 antibody CL488-68060Download protocol
Standard Protocols
Click here to view our Standard Protocols