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COPE Polyclonal antibody

COPE Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11457-1-AP

Print datasheet

Synonyms

Coatomer subunit epsilon, COPE, Epsilon coat protein, epsilon COP

验证数据展示

  • WB analysis of HEK-293 using 11457-1-AP

    WB analysis of HEK-293 using 11457-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 11457-1-AP

    IF Staining of HepG2 using 11457-1-AP

    Immunofluorescent analysis of HepG2 cells, using COPE antibody 11457-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human prostate cancer using 11457-1-AP

    IHC staining of human prostate cancer using 11457-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with COPE antibody included
  • IHC staining of human prostate cancer using 11457-1-AP

    IHC staining of human prostate cancer using 11457-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with COPE antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, PC-3 cells, A375 cells, HeLa cells, A431 cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy COPE Ready-To-Use IHC Kit. COPE primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11457-1-AP targets COPE in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human
Immunogen COPE fusion protein Ag2024 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coatomer protein complex, subunit epsilon
Synonyms Coatomer subunit epsilon, COPE, Epsilon coat protein, epsilon COP
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC017285
Gene Symbol COPE
Gene ID (NCBI) 11316
RRIDAB_11182386
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14579
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COPE antibody 11457-1-APDownload protocol
IHC protocol for COPE antibody 11457-1-APDownload protocol
IF protocol for COPE antibody 11457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols