Various lysates were subjected to SDS PAGE followed by western blot with 68118-1-Ig (BNIP3L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BNIP3L antibody (68118-1-Ig, Clone: 1F6A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.
