Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25805-1-AP (NF-M antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 25805-1-AP (NF-M antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25805-1-AP (NF-M antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 25805-1-AP (NF-M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

mouse brain tissue were subjected to SDS PAGE followed by western blot with 25805-1-AP (NEFM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M (25805-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

(blue)1x10^6 PC-12 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human NF-M (CL488-66396, Clone:2E3B12)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite®594 NF-M antibody (CL594-66396, Clone: 2E3B12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using KHC0045 (NF-M IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC0045 (NF-M IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using KHC0045 (NF-M IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using KHC0045 (NF-M IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Lysates of rat brain, mouse brain tissues and PC-12 cells were subjected to SDS PAGE followed by western blot with 66396-1-Ig (NEFM Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M (66396-1-Ig, Clone:2E3B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66396-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66396-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66396-1-PBS in a different storage buffer formulation.

Lysates of rat brain, mouse brain tissues and PC-12 cells were subjected to SDS PAGE followed by western blot with 66396-1-Ig (NEFM Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66396-1-PBS in a different storage buffer formulation.

1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M (66396-1-Ig, Clone:2E3B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66396-1-PBS in a different storage buffer formulation.

mouse brain and rat brain tissues were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

WB result of 20664-1-AP.

IP Result of anti-NF-M-Specific (IP:20664-1-AP, 4ug; Detection:20664-1-AP 1:1000) with rat brain tissue lysate 4000ug.

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 40x lens).

Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20664-1-AP (NF-M-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M-Specific (20664-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).