Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:300 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: CL555-phalloidin staining of F-actin. . This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 66037-1-Ig (ATP5A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human heart using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human heart using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.
IP result of anti-ATP5A1 (IP:66037-1-Ig, 5ug; Detection:66037-1-Ig 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.
1X10^6 HeLa cells were stained with 0.2 ug Anti-Human ATP5A1 (66037-1-Ig, Clone:1B10H3) and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or stained with 0.2 ug isotype control and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (blue). Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.
WB result of ATP5A1 antibody (66037-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP5A1 transfected HepG2 cells. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (66037-1-Ig, Clone: 1B10H3 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.