mouse brain tissues (heated at 100°C, unboided, heated at 37°C) were subjected to SDS PAGE followed by western blot with 12503-1-AP (Kir4.1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP Result of anti-Kir4.1 (IP:12503-1-AP, 4ug; Detection:12503-1-AP 1:500) with rat brain tissue lysate 4800ug.
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
