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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-10 protein

Activity

0.18-2.0 ng/mL

Specific Activity

minimally 7.00 x 106 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1145

Synonyms

CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF

Validation Data Gallery

  • Purity of recombinant human IL-10 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human IL-10 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

  • Recombinant human IL-10 (HZ-1145) induces dose-dependent proliferation of the MC/9 (mouse mast cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. MC/9 cells were treated with increasing concentration of recombinant IL-10 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.18-2.0 ng/mL.

    Recombinant human IL-10 (HZ-1145) induces dose-dependent proliferation of the MC/9 (mouse mast cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. MC/9 cells were treated with increasing concentration of recombinant IL-10 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.18-2.0 ng/mL.

  • Proteintech IL-10 (HZ-1145) demonstrates equivalent induction of proliferation and EC50 to leading competitors. IL-10 Induces dose-dependent proliferation of the MC/9 (mouse mast cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. MC/9 cells were treated with increasing concentration of recombinant IL-10 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.18-2.0 ng/mL.

    Proteintech IL-10 (HZ-1145) demonstrates equivalent induction of proliferation and EC50 to leading competitors. IL-10 Induces dose-dependent proliferation of the MC/9 (mouse mast cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. MC/9 cells were treated with increasing concentration of recombinant IL-10 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.18-2.0 ng/mL.

Technical Specifications

GeneID 3586
Species Human
Expression HEK293
Activity 0.18-2.0 ng/mL
Specific Activityminimally 7.00 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP22301
Molecular Mass 19 kDa reduced, 17 kDa non-reduced, non-disulfide bonded homodimer, glycosylated
Formulation 10mM Tris-HCl pH 7.4 + 150mM NaCl, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL-10 is a non-covalent homodimeric glycoprotein with an apparent molecular mass of 17 kDa. IL-10 is produced by a variety of mammalian cell types including T cells, B cells, macrophages, and keratinocytes monocytes. It is an anti-inflammatory cytokine with multiple roles in inflammation and immunoregulation (PMID: 19161426; 9162098).

Synonyms

CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF

Publications

SpeciesTitle
human

Stem Cell Res Ther

hPMSCs inhibit the expression of PD-1 in CD4+IL-10+ T cells and mitigate liver damage in a GVHD mouse model by regulating the crosstalk between Nrf2 and NF-κB signaling pathway.

Authors - Aiping Zhang
View Article

Cancer Immunol Immunother

Clinicopathological significance of peritumoral alveolar macrophages in patients with resected early-stage lung squamous cell carcinoma

Authors - Yu Tanaka
View Article
pig

Curr Issues Mol Biol

Transcriptomic Establishment of Pig Macrophage Polarization Signatures

Authors - Jing Li
View Article

Cancer Cell

Clearance of senescent macrophages ameliorates tumorigenesis in KRAS-driven lung cancer

Authors - Scott Haston
View Article
human

Cancer Lett

Hypoxia-induced TREM1 promotes mesenchymal-like states of glioma stem cells via alternatively activating tumor-associated macrophages

Authors - Minhai Dong
View Article
human

Gut Microbes

Microbiota-derived I3A protects the intestine against radiation injury by activating AhR/IL-10/Wnt signaling and enhancing the abundance of probiotics

Authors - Li-Wei Xie
View Article