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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-10 protein

Activity

0.18-2.0 ng/mL EC50

Specific Activity

7.5 x 10^6 IU/mg calibrated against IL-10 reference standard (NIBSC cat no: 93/722). Reference use only. Specific activity is not routinely tested

Species Reactivity

human,mouse

Purity

>95%

Cat No : HZ-1145

Print datasheet

Synonyms

CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF

Validation Data Gallery

  • Purity of recombinant human IL-10 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human IL-10 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

  • Proteintech IL-10 demonstrates equivalent induction of proliferation and EC50 to leading competitors. IL-10 Induces dose-dependent proliferation of the MC/9 (mouse mast cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. MC/9 cells were treated with increasing concentration of recombinant IL-10 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.18-2.0 ng/mL.

    Proteintech IL-10 demonstrates equivalent induction of proliferation and EC50 to leading competitors. IL-10 Induces dose-dependent proliferation of the MC/9 (mouse mast cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. MC/9 cells were treated with increasing concentration of recombinant IL-10 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.18-2.0 ng/mL.

  • Recombinant human IL-10 (HZ-1145) induces dose-dependent proliferation of the MC/9 (mouse mast cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. MC/9 cells were treated with increasing concentration of recombinant IL-10 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.18-2.0 ng/mL.

    Recombinant human IL-10 (HZ-1145) induces dose-dependent proliferation of the MC/9 (mouse mast cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. MC/9 cells were treated with increasing concentration of recombinant IL-10 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.18-2.0 ng/mL.

Technical Specifications

GeneID 3586
Species Human
Expression HEK293
Activity 0.18-2.0 ng/mL EC50
Specific Activity7.5 x 10^6 IU/mg calibrated against IL-10 reference standard (NIBSC cat no: 93/722). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP22301
Molecular Mass 17 kDa, non-disulfide bonded homodimer, glycosylated
Formulation 10mM Tris-HCl pH 7.4 + 150mM NaCl, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

IL-10 is a non-covalent homodimeric glycoprotein with an apparent molecular mass of 17 kDa. IL-10 is produced by a variety of mammalian cell types including T cells, B cells, macrophages, and keratinocytes monocytes. It is an anti-inflammatory cytokine with multiple roles in inflammation and immunoregulation (PMID: 19161426; 9162098).

Synonyms

CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF

Publications

SpeciesTitle
human

Stem Cell Res Ther

hPMSCs inhibit the expression of PD-1 in CD4+IL-10+ T cells and mitigate liver damage in a GVHD mouse model by regulating the crosstalk between Nrf2 and NF-κB signaling pathway.

Authors - Aiping Zhang
View Article