- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human IL-10 protein
Activity
0.18-2.0 ng/mL EC50
Specific Activity
7.5 x 10^6 IU/mg calibrated against IL-10 reference standard (NIBSC cat no: 93/722). Reference use only. Specific activity is not routinely tested
Species Reactivity
human,mouse
Purity
>95%
Validation Data Gallery
Technical Specifications
GeneID | 3586 |
Species | Human |
Expression | HEK293 |
Activity | 0.18-2.0 ng/mL EC50 |
Specific Activity | 7.5 x 10^6 IU/mg calibrated against IL-10 reference standard (NIBSC cat no: 93/722). Reference use only. Specific activity is not routinely tested |
Purity | >95% |
Endotoxin | <1 EU/μg |
Accession Number | P22301 |
Molecular Mass | 17 kDa, non-disulfide bonded homodimer, glycosylated |
Formulation | 10mM Tris-HCl pH 7.4 + 150mM NaCl, See Certificate of Analysis for details |
Species Reactivity | human,mouse |
Stability and Reconstitution
Stability and Storage | Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products. |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
Background
IL-10 is a non-covalent homodimeric glycoprotein with an apparent molecular mass of 17 kDa. IL-10 is produced by a variety of mammalian cell types including T cells, B cells, macrophages, and keratinocytes monocytes. It is an anti-inflammatory cytokine with multiple roles in inflammation and immunoregulation (PMID: 19161426; 9162098).
Synonyms
CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF
Publications
Species | Title |
---|---|
Stem Cell Res Ther hPMSCs inhibit the expression of PD-1 in CD4+IL-10+ T cells and mitigate liver damage in a GVHD mouse model by regulating the crosstalk between Nrf2 and NF-κB signaling pathway. |