• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human G-CSF protein


0.009-0.05 ng/mL EC50

Specific Activity

>1.00 x 108 IU/mg

Species Reactivity




Cat No : HZ-1207

Print datasheet


C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin

Technical Specifications

GeneID 1440
Species Human
Expression HEK293
Activity 0.009-0.05 ng/mL EC50
Specific Activity>1.00 x 108 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP09919
Molecular Mass 21 to 25 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).


Granulocyte colony-stimulating factor (G-CSF), also known as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils and trophoblast cells. G-CSF exerts its effects by binding to its receptor G-CSFR, in turn activating the JAK/STAT and Ras/Raf/MAP kinase pathways (PMID 9015216, 7509213, 9933603). G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy (PMID 7524753, 3264199). Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis (PMID 18524991).


C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin



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M2 macrophage-derived G-CSF promotes trophoblasts EMT, invasion and migration via activating PI3K/Akt/Erk1/2 pathway to mediate normal pregnancy.

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PLoS One

HIV-1 Tat Protein Induces Production of Proinflammatory Cytokines by Human Dendritic Cells and Monocytes/Macrophages through Engagement of TLR4-MD2-CD14 Complex and Activation of NF-κB Pathway.

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