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验证数据展示

  • WB analysis of A549 using 10828-1-AP (same clone as 10828-1-PBS)

    WB analysis of A549 using 10828-1-AP (same clone as 10828-1-PBS)

    WB result of c-MYC antibody (10828-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-MYC transfected A549 cells. This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

  • WB analysis using 10828-1-AP (same clone as 10828-1-PBS)

    WB analysis using 10828-1-AP (same clone as 10828-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 10828-1-AP (same clone as 10828-1-PBS)

    IP experiment of MCF-7 using 10828-1-AP (same clone as 10828-1-PBS)

    IP result of anti-c-MYC (IP:10828-1-AP, 4ug; Detection:10828-1-AP 1:1000) with MCF-7 cells lysate 1660 ug. This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 10828-1-AP (same clone as 10828-1-PBS)

    IHC staining of human ovary cancer using 10828-1-AP (same clone as 10828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10828-1-AP (c-MYC antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

  • IF Staining of Saos-2 using 10828-1-AP (same clone as 10828-1-PBS)

    IF Staining of Saos-2 using 10828-1-AP (same clone as 10828-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using c-MYC antibody (10828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 10828-1-AP (same clone as 10828-1-PBS)

    FC experiment of HeLa using 10828-1-AP (same clone as 10828-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human c-MYC (10828-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

  • FC experiment of NCCIT using 10828-1-AP (same clone as 10828-1-PBS)

    FC experiment of NCCIT using 10828-1-AP (same clone as 10828-1-PBS)

    1x10^6 NCCIT cells were intracellularly stained with 0.25 ug c-MYC Polyclonal antibody (10828-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 10828-1-AP (same clone as 10828-1-PBS)

    FC experiment of HeLa using 10828-1-AP (same clone as 10828-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-MYC Polyclonal antibody (10828-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

c-MYC Polyclonal antibody, PBS Only

c-MYC Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10828-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), IP, ELISA

别名

MYC, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39, MRTL

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

10828-1-PBS targets c-MYC in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 c-MYC fusion protein Ag1263 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-myc myelocytomatosis viral oncogene homolog (avian)
别名 MYC, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39, MRTL
计算分子量 49 kDa
观测分子量 62-65 kDa, 50 kDa
GenBank蛋白编号BC000141
基因名称 MYC
Gene ID (NCBI) 4609
RRIDAB_2148585
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01106
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Function

c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.

Tissue specificity

c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.

Involvement in disease

·         Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.

·         Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.

Isoforms

There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct. The 50kDa band recognized by antibody is the native form of MYC, while the other bands, between 60-70kDa, are the phosphorylated form of MYC (PMID: 12189186).

Post-translational modifications

c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.

Cellular localization

c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.