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c-MYC Polyclonal antibody
c-MYC Polyclonal Antibody for FC, IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (6)
Applications
FC, IF/ICC, IP, WB, ELISA and More (4)
Conjugate
Unconjugated
577
验证数据展示
经过测试的应用
Positive WB detected in | HT-29 cells, Jurkat cells, HeLa cells, A549 cells, HL-60 cells, human placenta tissue, Raji cells, SH-SY5Y cells, HepG2 cells, BxPC-3 cells, SW 1990 cells, Jurkat cells and Raji cells. |
Positive IP detected in | MCF-7 cells |
Positive IF detected in | Saos-2 cells, HEK-293 cells, U2OS cells |
Positive FC detected in | HeLa cells |
This antibody is not for the detetion of MYC-tag.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10828-1-AP targets c-MYC in WB, IP, IF, FC, IHC, EMSA, CoIP, chIP, ELISA applications and shows reactivity with human samples.
Tested Applications | FC, IF/ICC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, IHC, EMSA, CoIP, chIP |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, chicken, sheep, P. lilacinum, pig |
Immunogen | c-MYC fusion protein Ag1263 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | v-myc myelocytomatosis viral oncogene homolog (avian) |
Synonyms | bHLHe39, c Myc, c-Myc, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64 |
Calculated Molecular Weight | 49 kDa |
Observed Molecular Weight | 62-65 kDa, 50 kDa |
GenBank Accession Number | BC000141 |
Gene Symbol | MYC |
Gene ID (NCBI) | 4609 |
RRID | AB_2148585 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P01106 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Function
c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.
Tissue specificity
c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.
Involvement in disease
· Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.
· Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.
Isoforms
There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct.
Post-translational modifications
c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.
Cellular localization
c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.
实验方案
Product Specific Protocols | |
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WB protocol for c-MYC antibody 10828-1-AP | Download protocol |
IF protocol for c-MYC antibody 10828-1-AP | Download protocol |
IP protocol for c-MYC antibody 10828-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration
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Signal Transduct Target Ther Overexpression of CIP2A is associated with poor prognosis in multiple myeloma. | ||
Gastroenterology Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis | ||
Cancer Cell p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.
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Cell Metab Efferocytosis induces macrophage proliferation to help resolve tissue injury.
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