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c-MYC Polyclonal antibody

c-MYC Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10828-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (6)

应用

WB, IF/ICC, FC (Intra), IP, ELISA and More (5)

MYC, Myc proto oncogene protein, MRTL, Class E basic helix-loop-helix protein 39, c Myc

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHT-29 cells, Jurkat cells, HeLa cells, A549 cells, HL-60 cells, human placenta tissue, Raji cells, SH-SY5Y cells, HepG2 cells, BxPC-3 cells, SW 1990 cells, Jurkat cells and Raji cells.
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSaos-2 cells, HEK-293 cells, U2OS cells
Positive FC (Intra) detected inHeLa cells, NCCIT cells

This antibody is not for the detetion of MYC-tag.

Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10828-1-AP targets c-MYC in WB, IF/ICC, FC (Intra), IP, CoIP, chIP, RIP, ELISA, EMSA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IF, IP, CoIP, chIP, RIP, EMSA
经测试反应性 human
文献引用反应性human, mouse, rat, pig, chicken, sheep, p. lilacinum
免疫原 c-MYC fusion protein Ag1263 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-myc myelocytomatosis viral oncogene homolog (avian)
别名 MYC, Myc proto oncogene protein, MRTL, Class E basic helix-loop-helix protein 39, c Myc
计算分子量 49 kDa
观测分子量 62-65 kDa, 50 kDa
GenBank蛋白编号BC000141
基因名称 MYC
Gene ID (NCBI) 4609
RRIDAB_2148585
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01106
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Function

c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.

Tissue specificity

c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.

Involvement in disease

·         Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.

·         Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.

Isoforms

There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct. The 50kDa band recognized by antibody is the native form of MYC, while the other bands, between 60-70kDa, are the phosphorylated form of MYC (PMID: 12189186).

Post-translational modifications

c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.

Cellular localization

c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.


实验方案

Product Specific Protocols
WB protocol for c-MYC antibody 10828-1-APDownload protocol
IHC protocol for c-MYC antibody 10828-1-APDownload protocol
IF protocol for c-MYC antibody 10828-1-APDownload protocol
IP protocol for c-MYC antibody 10828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

RIC-seq for global in situ profiling of RNA-RNA spatial interactions.

Authors - Zhaokui Cai
mouseWB

Signal Transduct Target Ther

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

Authors - Jinpeng Du
  • KD Validated
humanWB

Signal Transduct Target Ther

Overexpression of CIP2A is associated with poor prognosis in multiple myeloma.

Authors - Xuewen Liu
humanWB,IF

Gastroenterology

Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis

Authors - Xiao-Mei Yang
humanWB

Cancer Cell

p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Authors - Alessandro Carugo
  • KD Validated
WB

Cell Metab

Efferocytosis induces macrophage proliferation to help resolve tissue injury.

Authors - Brennan D Gerlach
  • KD Validated
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