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验证数据展示

  • WB analysis using 14881-1-AP (same clone as 14881-1-PBS)

    WB analysis using 14881-1-AP (same clone as 14881-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 14881-1-AP (same clone as 14881-1-PBS)

    IHC staining of mouse brain using 14881-1-AP (same clone as 14881-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14881-1-AP (YWHAZ antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YWHAZ antibody included
  • IHC staining of human skin cancer using 14881-1-AP (same clone as 14881-1-PBS)

    IHC staining of human skin cancer using 14881-1-AP (same clone as 14881-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14881-1-AP (YWHAZ antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YWHAZ antibody included
  • Featured Product
  • KD/KO Validated

YWHAZ Polyclonal antibody, PBS Only

YWHAZ Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14881-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

14-3-3, 14-3-3 protein zeta/delta, KCIP 1, KCIP-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy YWHAZ Ready-To-Use IHC Kit. YWHAZ primary antibody included.

产品信息

14881-1-PBS targets YWHAZ in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6654

Product name: Recombinant human YWHAZ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-245 aa of BC003623

Sequence: MDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEGGEN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
别名 14-3-3, 14-3-3 protein zeta/delta, KCIP 1, KCIP-1
计算分子量 28 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC003623
基因名称 YWHAZ
Gene ID (NCBI) 7534
RRIDAB_2218248
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP63104
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAZ.

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