验证数据展示
经过测试的应用
| Positive WB detected in | mouse bone marrow, mouse spleen tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33153-1-AP targets YM-1+YM-2 in WB, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Ag39745 Product name: Recombinant mouse CHIL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-368 aa of NM_009892 Sequence: YQLMCYYTSWAKDRPIEGSFKPGNIDPCLCTHLIYAFAGMQNNEITYTHEQDLRDYEALNGLKDKNTELKTLLAIGGWKFGPAPFSAMVSTPQNRQIFIQSVIRFLRQYNFDGLNLDWQYPGSRGSPPKDKHLFSVLVKEMRKAFEEESVEKDIPRLLLTSTGAGIIDVIKSGYKIPELSQSLDYIQVMTYDLHDPKDGYTGENSPLYKSPYDIGKSADLNVDSIISYWKDHGAASEKLIVGFPAYGHTFILSDPSKTGIGAPTISTGPPGKYTDESGLLAYYEVCTFLNEGATEVWDAPQEVPYAYQGNEWVGYDNVRSFKLKAQWLKDNNLGGAVVWPLDMDDFSGSFCHQRHFPLTSTLKGDLNIHSASCKGPY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chitinase 3-like 3 |
| 别名 | Beta-N-acetylhexosaminidase Ym1, Chi3l3, Chil3, CHIL4, Chitinase-3-like protein 3 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 44 kDa |
| GenBank蛋白编号 | NM_009892 |
| 基因名称 | Chi3l3 |
| Gene ID (NCBI) | 12655 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O35744 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Chitinase-like protein 3 (Chil3), also called ECF-L or Ym1, is a member of chitinase-like protein family which has chemotactic activity for eosinophils (PMID: 27256220). CHIL3 promotes NSC self-renewal and commitment to the neuronal lineage. Endogenous expression of CHIL3 is observed in the neurogenic niche of the mouse brain and retina and in lesions of those injury models (PMID: 36368330). Chitinase-like protein 4 (Chil4), also called Ym2, regulates regeneration of the injured OE in vivo in both male and female mice and cell proliferation/differentiation in OE colonies in vitro (PMID: 34016714). This antibody detects both YM1 and YM2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for YM-1+YM-2 antibody 33153-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
