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验证数据展示

  • WB analysis of L02 using 16462-1-AP (same clone as 16462-1-PBS)

    WB analysis of L02 using 16462-1-AP (same clone as 16462-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • IP experiment of L02 using 16462-1-AP (same clone as 16462-1-PBS)

    IP experiment of L02 using 16462-1-AP (same clone as 16462-1-PBS)

    IP result of anti-XPA (IP:16462-1-AP, 4ug; Detection:16462-1-AP 1:300) with L02 cells lysate 3000 ug. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16462-1-AP (same clone as 16462-1-PBS)

    IF Staining of HepG2 using 16462-1-AP (same clone as 16462-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XPA antibody (16462-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 16462-1-AP (same clone as 16462-1-PBS)

    FC experiment of HepG2 using 16462-1-AP (same clone as 16462-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XPA (16462-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

XPA Polyclonal antibody, PBS Only

XPA Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 16462-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy XPA Ready-To-Use IHC Kit. XPA primary antibody included.

产品信息

16462-1-PBS targets XPA in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 XPA fusion protein Ag9536 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 xeroderma pigmentosum, complementation group A
别名
计算分子量374aa,42 kDa; 273aa,31 kDa
观测分子量 40 kDa
GenBank蛋白编号BC014965
基因名称 XPA
Gene ID (NCBI) 7507
RRIDAB_2878261
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23025
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

XPA, also named as DNA repair protein complementing XP-A cells, is a 272 amino acid protein, which belongs to the XPA family. XPA is expressed in various cell lines and in skin fibroblasts. XPA is involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. XPA is required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. The calculated molecular weight of XPA is 31 kDa, but modified XPA protein is about 40 kDa (PMID: 17848622).