CoraLite® Plus 488-conjugated WNK1 Monoclonal antibody
WNK1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B6A10
验证数据展示
经过测试的应用
Positive FC detected in | HEK-293T cells |
Planning an IHC experiment? We recommend our IHCeasy WNK1 Ready-To-Use IHC Kit. WNK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68149-1-Ig
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-68149 targets WNK1 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | FC (Intra) |
Tested Reactivity | Human, Mouse, Rat |
Immunogen | WNK1 fusion protein Ag28044 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | WNK lysine deficient protein kinase 1 |
Synonyms | Erythrocyte 65 kDa protein, HSAN2, HSN2, hWNK1, KDP, KIAA0344, Kinase deficient protein, p65, PHA2C, PRKWNK1, PSK, WNK1 |
Calculated Molecular Weight | 251 kDa |
Observed Molecular Weight | 250 kDa |
GenBank Accession Number | BC021121 |
Gene Symbol | WNK1 |
Gene ID (NCBI) | 65125 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9H4A3 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WNK kinase is a unique serine-threonine kinase in which the lysine (K) residue in the catalytic domain, that is found in the majority of kinases is not conserved. In mammals, there are four WNK kinase family members (WNK1-4). WNK1 has 6 isoforms, such as long form WNK1 (L-WNK1, 250 kDa, strong expression in dorsal root ganglia and spinal cord) ; kidney-specific isoform (KS-WNK1, 207 kDa, lacks a kinase domain).KS-WNK1 cannot phosphorylate substrates and instead interacts with and modulates the actions of other WNKs via a carboxyl terminal HQ domain.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 WNK1 antibody CL488-68149 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |