验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | HEK-293T cells |
| Positive FC detected in | HEK-293T cells |
Planning an IHC experiment? We recommend our IHCeasy WNK1 Ready-To-Use IHC Kit. WNK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68149-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68149 targets WNK1 in IF/ICC, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 | WNK1 fusion protein Ag28044 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | WNK lysine deficient protein kinase 1 |
| 别名 | Erythrocyte 65 kDa protein, HSAN2, HSN2, hWNK1, KDP, KIAA0344, Kinase deficient protein, p65, PHA2C, PRKWNK1, PSK, WNK1 |
| 计算分子量 | 251 kDa |
| 观测分子量 | 250 kDa |
| GenBank蛋白编号 | BC021121 |
| 基因名称 | WNK1 |
| Gene ID (NCBI) | 65125 |
| RRID | AB_3084420 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9H4A3 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WNK kinase is a unique serine-threonine kinase in which the lysine (K) residue in the catalytic domain, that is found in the majority of kinases is not conserved. In mammals, there are four WNK kinase family members (WNK1-4). WNK1 has 6 isoforms, such as long form WNK1 (L-WNK1, 250 kDa, strong expression in dorsal root ganglia and spinal cord) ; kidney-specific isoform (KS-WNK1, 207 kDa, lacks a kinase domain).KS-WNK1 cannot phosphorylate substrates and instead interacts with and modulates the actions of other WNKs via a carboxyl terminal HQ domain.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 WNK1 antibody CL488-68149 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |