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验证数据展示

  • IHC staining of mouse colon using 14658-1-AP (same clone as 14658-1-PBS)

    IHC staining of mouse colon using 14658-1-AP (same clone as 14658-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14658-1-AP (WIBG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14658-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 14658-1-AP (same clone as 14658-1-PBS)

    IF Staining of U2OS using 14658-1-AP (same clone as 14658-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using WIBG antibody (14658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14658-1-PBS in a different storage buffer formulation.

WIBG Polyclonal antibody, PBS Only

WIBG Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 14658-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

14658-1-PBS targets WIBG in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6276

Product name: Recombinant human WIBG protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-204 aa of BC006135

Sequence: MEAAGSPAATETGKYIASTQRPDGTWRKQRRVKEGYVPQEEVPVYENKYVKFFKSKPELPPGLSPEATAPVTPSRPEGGEPGLSKTAKRNLKRKEKRRQQQEKGEAEALSRTLDKVSLEETAQLPSAPQGSRAAPTAASDQPDSAATTEKAKKIKNLKKKLRQVEELQQRIQAGEVSQPSKEQLEKLARRRALEEELEDLELGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 within bgcn homolog (Drosophila)
别名
计算分子量 23 kDa
GenBank蛋白编号BC006135
基因名称 WIBG
Gene ID (NCBI) 84305
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRP8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein WIBG homolog, also known as PYM1 (Partner of Y14 and mago), is a cytoplasmic RNA-binding protein, that can interact with the ribosome and EJC, may act as an EJC destabilizing factor, preventing EJC accumulation at positions away from exon-exon junctions (non-canonical position).

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