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WIBG Polyclonal antibody

WIBG Polyclonal Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 14658-1-AP

Print datasheet

Synonyms

Partner of Y14 and mago, Protein wibg homolog, PYM, WIBG

验证数据展示

  • IHC staining of mouse colon using 14658-1-AP

    IHC staining of mouse colon using 14658-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14658-1-AP (WIBG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 14658-1-AP

    IF Staining of U2OS using 14658-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using WIBG antibody (14658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14658-1-AP targets WIBG in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, ELISA
Tested Reactivity human, mouse, rat
Immunogen WIBG fusion protein Ag6276 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name within bgcn homolog (Drosophila)
Synonyms Partner of Y14 and mago, Protein wibg homolog, PYM, WIBG
Calculated Molecular Weight 23 kDa
GenBank Accession NumberBC006135
Gene Symbol WIBG
Gene ID (NCBI) 84305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRP8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYM is a cytoplasmic RNA-binding protein, which has been identified as an interacting partner of Mago-Y14. PYM interacts directly with Mago-Y14 by means of its N-terminal domain. Mago and Y14 are core components of the exon junction complex (EJC).

实验方案

Product Specific Protocols
IHC protocol for WIBG antibody 14658-1-APDownload protocol
IF protocol for WIBG antibody 14658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols