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验证数据展示

  • WB analysis using 17673-1-AP (same clone as 17673-1-PBS)

    WB analysis using 17673-1-AP (same clone as 17673-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 17673-1-AP (same clone as 17673-1-PBS)

    IP experiment of HeLa using 17673-1-AP (same clone as 17673-1-PBS)

    IP result of anti-VPS4A/B (IP:17673-1-AP, 4ug; Detection:17673-1-AP 1:3000) with HeLa cells lysate 1360 ug. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 17673-1-AP (same clone as 17673-1-PBS)

    FC experiment of A431 using 17673-1-AP (same clone as 17673-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug VPS4A/B Polyclonal antibody (17673-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

VPS4A/B Polyclonal antibody, PBS Only

VPS4A/B Polyclonal Antibody for WB, FC (Intra), IP, Indirect ELISA
Cat No. 17673-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), IP, Indirect ELISA

别名

VPS4B, Vacuolar protein sorting-associated protein 4B, SKD1, Protein SKD1, MIG1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

17673-1-PBS targets VPS4A/B in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11610

Product name: Recombinant human VPS4B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-444 aa of BC039574

Sequence: MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vacuolar protein sorting 4 homolog B (S. cerevisiae)
别名 VPS4B, Vacuolar protein sorting-associated protein 4B, SKD1, Protein SKD1, MIG1
计算分子量 444 aa, 49 kDa
观测分子量 50 kDa
GenBank蛋白编号BC039574
基因名称 VPS4B
Gene ID (NCBI) 9525
RRIDAB_2215504
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75351
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. Human VPS4 has two orthologous proteins named VPS4A and VPS4B. This antibody can recognize both VPS4A and VPS4B due to their high homology.

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