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验证数据展示

  • WB analysis using 67318-1-Ig

    WB analysis using 67318-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67318-1-Ig (UBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

UBP1 Monoclonal antibody

UBP1 Monoclonal Antibody for WB, ELISA
Cat No. 67318-1-Ig

产品说明书

CloneNo. 1B10H3

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

DKFZp686L1745, LBP 1a, LBP 1B, LBP1, LBP1A, LBP1B, Transcription factor LBP 1, UBP1, Upstream binding protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, LNCaP cells, K-562 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67318-1-Ig targets UBP1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag5010

Product name: Recombinant human UBP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 280-540 aa of BC047235

Sequence: IEDAVEHEQKKSSKRTLPADYGDSLAKRGSCSPWPDAPTAYVNNSPSPAPTFTSPQQSTCSVPDSNSSSPNHQGDGASQTSGEQIQPSATIQETQQWLLKNRFSSYTRLFSNFSGADLLKLTKEDLVQICGAADGIRLYNSLKSRSVRPRLTIYVCREQPSSTVLQGQQQAASSASENGSGAPYVYHAIYLEEMIASEVARKLALVFNIPLHQINQVYRQGPTGIHILVSDQMVQNFQDESCFLFSTVKAESSDGIHIILK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 upstream binding protein 1 (LBP-1a)
别名 DKFZp686L1745, LBP 1a, LBP 1B, LBP1, LBP1A, LBP1B, Transcription factor LBP 1, UBP1, Upstream binding protein 1
计算分子量 60 kDa
观测分子量 65-68 kDa
GenBank蛋白编号BC047235
基因名称 UBP1
Gene ID (NCBI) 7342
RRIDAB_2882578
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9NZI7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBP1, also known as Transcription factor LBP-1, is a 540 amino acid protein, which is expressed in adrenal tissue, JEG-3, NCI-H295A, Hep-G2 and HeLa cell lines. UBP1 localizes in nuclear and can be induced by HIV-1 infection of lymphocytes. UBP1 functions as a transcriptional activator in a promoter context-dependent manner and modulates the placental expression of CYP11A1. UBP1 is involved in regulation of the alpha-globin gene in erythroid cells and represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.

实验方案

Product Specific Protocols
WB protocol for UBP1 antibody 67318-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols