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验证数据展示

  • WB analysis using 87282-1-RR (same clone as 87282-1-PBS)

    WB analysis using 87282-1-RR (same clone as 87282-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87282-1-RR (UBE2O antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87282-1-PBS in a different storage buffer formulation.

  • WB analysis using 87282-1-RR (same clone as 87282-1-PBS)

    WB analysis using 87282-1-RR (same clone as 87282-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87282-1-RR (UBE2O antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87282-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 87282-1-RR (same clone as 87282-1-PBS)

    IF Staining of A431 using 87282-1-RR (same clone as 87282-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using UBE2O antibody (87282-1-RR, Clone: 252545E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87282-1-PBS in a different storage buffer formulation.

  • Affinity of 87282-1-RR

    Affinity of 87282-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87282-1-RR against Human UBE2O were performed. The affinity constant is 16.1 nM.

UBE2O Recombinant monoclonal antibody, PBS Only

UBE2O Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87282-1-PBS

产品说明书

CloneNo. 252545E2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

(E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, E2-230K, EC:2.3.2.24, KIAA1734

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87282-1-PBS targets UBE2O in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8580

Product name: Recombinant human UBE2O protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-343 aa of BC007822

Sequence: SSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2O
别名 (E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, E2-230K, EC:2.3.2.24, KIAA1734
计算分子量 1292 aa, 141 kDa
观测分子量 200 kDa
GenBank蛋白编号BC007822
基因名称 UBE2O
Gene ID (NCBI) 63893
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9C0C9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBE2O, also named as KIAA1734, belongs to the ubiquitin-conjugating enzyme family. It catalyzes the covalent attachment of ubiquitin to other proteins. UBE2O has a calculated molecular mass of 141 kDa, and many bands can be detected as 140-170 kDa and 200-230 kDa (PMID: 28774900, 23455153, 28774922, 11311559).

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