• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HEK-293 using 55457-1-AP (same clone as 55457-1-PBS)

    WB analysis of HEK-293 using 55457-1-AP (same clone as 55457-1-PBS)

    WB result of UBE2E1 antibody (55457-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE2E1 transfected HEK-293 cells. This data was developed using the same antibody clone with 55457-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 55457-1-AP (same clone as 55457-1-PBS)

    IHC staining of human breast cancer using 55457-1-AP (same clone as 55457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55457-1-AP (UBE2E1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55457-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 55457-1-AP (same clone as 55457-1-PBS)

    IHC staining of human breast cancer using 55457-1-AP (same clone as 55457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55457-1-AP (UBE2E1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55457-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 55457-1-AP (same clone as 55457-1-PBS)

    IF Staining of A431 using 55457-1-AP (same clone as 55457-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using UBE2E1 antibody (55457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 55457-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

UBE2E1 Polyclonal antibody, PBS Only

UBE2E1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 55457-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

UBCH6, UBE2E 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

55457-1-PBS targets UBE2E1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast)
别名 UBCH6, UBE2E 1
计算分子量 21 kDa
观测分子量21-27 kDa
GenBank蛋白编号NM_003341
基因名称 UBE2E1
Gene ID (NCBI) 7324
RRIDAB_2918357
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51965
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBE2E1 is a class III E2 ubiquitin conjugating enzyme that belongs to the UBE2E family of E2s, comprising UBE2E1/UBCH6, UBE2E2/UBCH8, and UBE2E3/UBCH9. UBE2E family members share a highly conserved UBC domain but are distinguished from one another by their unique N-terminal extensions. UbE2E1 contains a 50-residue unstructured N-terminal extension rich in serine and lysine residues, which has been shown to interact with USP7 and is essential for USP7-mediated UbE2E1 stabilization. In addition to its ubiquitin conjugating activity, UBE2E1 can also act as an ISG15 conjugating enzyme in vitro, although this activity has not been shown to occur in vivo. UBE2E1 can be covalently modified with either ISG15 or ubiquitin, both of which interfere with the ubiquitin conjugating activity of UBE2E1. UBE2E1 has also been reported to monoubiquitinate histone H2A at K119 in concert with the PRC1 E3 ligase complex. Full-length UBE2E1 is reported to be a dimer, with dimerization mediated by interaction of residues 21-27 from one molecule with the core domain of another molecule, as well as by contacts between a1 of the two interacting molecules. (PMID: 28073915, PMID: 30282802, PMID: 23871895)