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验证数据展示

  • WB analysis of K-562 using 67588-1-Ig

    WB analysis of K-562 using 67588-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67588-1-Ig (UBAP2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67588-1-Ig

    WB analysis of HEK-293 using 67588-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67588-1-Ig (UBAP2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

UBAP2L Monoclonal antibody

UBAP2L Monoclonal Antibody for WB, ELISA
Cat No. 67588-1-Ig

产品说明书

CloneNo. 1E3D1

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, ELISA

别名

Protein NICE-4, Protein NICE 4, NICE4, NICE 4, KIAA0144

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, LNCaP cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67588-1-Ig targets UBAP2L in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 UBAP2L fusion protein Ag30125 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin associated protein 2-like
别名 Protein NICE-4, Protein NICE 4, NICE4, NICE 4, KIAA0144
计算分子量 115 kDa
观测分子量 140-160 kDa
GenBank蛋白编号BC003170
基因名称 UBAP2L
Gene ID (NCBI) 9898
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14157
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin-associated protein 2-like (UBAP2L) is a highly conserved protein with an N-terminal ubiquitin-associated (UBA) domain involved in the ubiquitin-proteasome system and aggregate formation induced by proteasome inhibitors. It was originally identified as a human sperm protein that interacts with zona pellucida 3 in human eggs. It can interact with BIM1 to form a complex that modulates hematopoietic stem cell activity. Recent studies have confirmed that UBAP2L function as an oncogene and is associated with various types of cancer, including prostate cancer, glioma, hepatocellular carcinoma (HCC), and colorectal carcinoma. (PMID: 31114027, PMID: 29196913)

实验方案

Product Specific Protocols
WB protocol for UBAP2L antibody 67588-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols