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验证数据展示

  • WB analysis using 28725-1-AP (same clone as 28725-1-PBS)

    WB analysis using 28725-1-AP (same clone as 28725-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28725-1-AP (TRIM58 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28725-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 28725-1-AP (same clone as 28725-1-PBS)

    WB analysis of PC-3 using 28725-1-AP (same clone as 28725-1-PBS)

    WB result of TRIM58 antibody (28725-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM58 transfected PC-3 cells. This data was developed using the same antibody clone with 28725-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRIM58 Polyclonal antibody, PBS Only

TRIM58 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 28725-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

BIA2, Protein BIA2, TRIM, tripartite motif containing 58

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

28725-1-PBS targets TRIM58 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30014

Product name: Recombinant human TRIM58 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 120-330 aa of NM_015431

Sequence: AGPEHRTHRTAPLQEAAGSYQVKLQMALELMRKELEDALTQEANVGKKTVIWKEKVEMQRQRFRLEFEKHRGFLAQEEQRQLRRLEAEERATLQRLRESKSRLVQQSKALKELADELQERCQRPALGLLEGVRGVLSRSKAVTRLEAENIPMELKTACCIPGRRELLRKFQVDVKLDPATAHPSLLLTADLRSVQDGEPWRDVPNNPERFD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 58
别名 BIA2, Protein BIA2, TRIM, tripartite motif containing 58
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号NM_015431
基因名称 TRIM58
Gene ID (NCBI) 25893
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NG06
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM58 (Tripartite motif-containing protein 58) is an E3 ubiquitin ligase induced during late erythropoiesis. It can directly bind and ubiquitinate the intermediate chain of the microtubule motor dynein (DYNC1LI1/DYNC1LI2), stimulating the degradation of the dynein holoprotein complex. May participate in the erythroblast enucleation process through regulation of nuclear polarization (PMID: 25241935).

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