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验证数据展示

  • WB analysis using 28725-1-AP

    WB analysis using 28725-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28725-1-AP (TRIM58 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TRIM58 Polyclonal antibody

TRIM58 Polyclonal Antibody for WB, ELISA
Cat No. 28725-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

tripartite motif containing 58, TRIM, Protein BIA2, BIA2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28725-1-AP targets TRIM58 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 TRIM58 fusion protein Ag30014 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 58
别名 tripartite motif containing 58, TRIM, Protein BIA2, BIA2
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号NM_015431
基因名称 TRIM58
Gene ID (NCBI) 25893
RRIDAB_3669668
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NG06
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM58 (Tripartite motif-containing protein 58) is an E3 ubiquitin ligase induced during late erythropoiesis. It can directly bind and ubiquitinate the intermediate chain of the microtubule motor dynein (DYNC1LI1/DYNC1LI2), stimulating the degradation of the dynein holoprotein complex. May participate in the erythroblast enucleation process through regulation of nuclear polarization (PMID: 25241935).

实验方案

Product Specific Protocols
WB protocol for TRIM58 antibody 28725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols