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Species Reactivity
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Species Reactivity
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0
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验证数据展示

  • WB analysis using 11741-1-AP (same clone as 11741-1-PBS)

    WB analysis using 11741-1-AP (same clone as 11741-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11741-1-AP (TPX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11741-1-AP (same clone as 11741-1-PBS)

    IHC staining of human colon cancer using 11741-1-AP (same clone as 11741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPX2 antibody included
  • IHC staining of human prostate cancer using 11741-1-AP (same clone as 11741-1-PBS)

    IHC staining of human prostate cancer using 11741-1-AP (same clone as 11741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11741-1-AP (TPX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPX2 antibody included
  • IHC staining of human colon cancer using 11741-1-AP (same clone as 11741-1-PBS)

    IHC staining of human colon cancer using 11741-1-AP (same clone as 11741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPX2 antibody included
  • IP experiment of HeLa using 11741-1-AP (same clone as 11741-1-PBS)

    IP experiment of HeLa using 11741-1-AP (same clone as 11741-1-PBS)

    IP result of anti-TPX2 (IP:11741-1-AP, 4ug; Detection:11741-1-AP 1:800) with HeLa cells lysate 880ug. This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11741-1-AP (same clone as 11741-1-PBS)

    IF Staining of HeLa using 11741-1-AP (same clone as 11741-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11741-1-AP (TPX2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11741-1-AP (same clone as 11741-1-PBS)

    IF Staining of HepG2 using 11741-1-AP (same clone as 11741-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TPX2 antibody (11741-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TPX2 Polyclonal antibody, PBS Only

TPX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11741-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TPX2 Ready-To-Use IHC Kit. TPX2 primary antibody included.

产品信息

11741-1-PBS targets TPX2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TPX2 fusion protein Ag2334 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TPX2, microtubule-associated, homolog (Xenopus laevis)
别名 DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2
计算分子量 747 aa, 86 kDa
观测分子量 100 kDa
GenBank蛋白编号BC020207
基因名称 TPX2
Gene ID (NCBI) 22974
RRIDAB_2208895
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9ULW0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).