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验证数据展示

  • WB analysis using 13343-1-AP (same clone as 13343-1-PBS)

    WB analysis using 13343-1-AP (same clone as 13343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • WB analysis of A431 using 13343-1-AP (same clone as 13343-1-PBS)

    WB analysis of A431 using 13343-1-AP (same clone as 13343-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 13343-1-AP (same clone as 13343-1-PBS)

    IF Staining of HepG2 using 13343-1-AP (same clone as 13343-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13343-1-AP (TPSAB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 13343-1-AP (same clone as 13343-1-PBS)

    IP experiment of mouse liver using 13343-1-AP (same clone as 13343-1-PBS)

    IP result of anti-TPSAB1 (IP:13343-1-AP, 4ug; Detection:13343-1-AP 1:800) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 13343-1-AP (same clone as 13343-1-PBS)

    IHC staining of human tonsillitis using 13343-1-AP (same clone as 13343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPSAB1 antibody included
  • IF Staining of mouse skin using 13343-1-AP (same clone as 13343-1-PBS)

    IF Staining of mouse skin using 13343-1-AP (same clone as 13343-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TPSAB1 antibody (13343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • FC experiment of U-937 using 13343-1-AP (same clone as 13343-1-PBS)

    FC experiment of U-937 using 13343-1-AP (same clone as 13343-1-PBS)

    1X10^6 U-937 cells were intracellularly stained with 0.2 ug Anti-Human TPSAB1 (13343-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 13343-1-AP (same clone as 13343-1-PBS)

    IHC staining of human tonsillitis using 13343-1-AP (same clone as 13343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPSAB1 antibody included

TPSAB1 Polyclonal antibody, PBS Only

TPSAB1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA
Cat No. 13343-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

别名

EC:3.4.21.59, MCP7, TPS1, TPS2, TPSB1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TPSAB1 Ready-To-Use IHC Kit. TPSAB1 primary antibody included.

产品信息

13343-1-PBS targets TPSAB1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4167

Product name: Recombinant human TPSAB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-275 aa of BC028059

Sequence: MLSLLLLALPVLASPAYAAPAPVQALQQAGIVGGQEAPRSKWPWQVSLRVRDRYWMHFCGGSLIHPQWVLTAAHCLGPDVKDLATLRVQLREQHLYYQDQLLPVSRIIVHPQFYIIQTGADIALLELEEPVNISSRVHTVMLPPASETFPPGMPCWVTGWGDVDNDEPLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIIRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWDEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tryptase alpha/beta 1
别名 EC:3.4.21.59, MCP7, TPS1, TPS2, TPSB1
计算分子量 275 aa, 31 kDa
观测分子量 32-38 kDa
GenBank蛋白编号BC028059
基因名称 TPSAB1
Gene ID (NCBI) 7177
RRIDAB_2206197
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15661
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C