Various lysates were subjected to SDS PAGE followed by western blot with 28477-1-AP (TPM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28477-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28477-1-AP (TPM1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28477-1-PBS in a different storage buffer formulation.