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验证数据展示

  • WB analysis using 19925-1-AP (same clone as 19925-1-PBS)

    WB analysis using 19925-1-AP (same clone as 19925-1-PBS)

    Boiled and unboiled HeLa cell lysates were subjected to SDS PAGE followed by western blot with 19925-1-AP (TMEM175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • WB analysis using 19925-1-AP (same clone as 19925-1-PBS)

    WB analysis using 19925-1-AP (same clone as 19925-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19925-1-AP (TMEM175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 19925-1-AP (same clone as 19925-1-PBS)

    IHC staining of mouse brain using 19925-1-AP (same clone as 19925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19925-1-AP (TMEM175 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 19925-1-AP (same clone as 19925-1-PBS)

    IHC staining of mouse brain using 19925-1-AP (same clone as 19925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19925-1-AP (TMEM175 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TMEM175 Polyclonal antibody, PBS Only

TMEM175 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 19925-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Transmembrane protein 175, hTMEM175

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

19925-1-PBS targets TMEM175 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13890

Product name: Recombinant human TMEM175 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 222-313 aa of BC005158

Sequence: YVSKVTGWCRDRLLGHREPSAHPVEVFSFDLHEPLSKERVEAFSDGVYAIVATLLILDICEDNVPDPKDVKERFSGSLVAALSATGPRFLAY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 175
别名 Transmembrane protein 175, hTMEM175
计算分子量 504 aa, 56 kDa
观测分子量41-45 kDa, 54-60 kDa
GenBank蛋白编号BC005158
基因名称 TMEM175
Gene ID (NCBI) 84286
RRIDAB_10666166
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BSA9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.

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