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验证数据展示

  • WB analysis using 68495-1-Ig (same clone as 68495-1-PBS)

    WB analysis using 68495-1-Ig (same clone as 68495-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68495-1-Ig (TJAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68495-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 68495-1-Ig (same clone as 68495-1-PBS)

    WB analysis of HepG2 using 68495-1-Ig (same clone as 68495-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68495-1-Ig (TJAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68495-1-PBS in a different storage buffer formulation.

TJAP1 Monoclonal antibody, PBS Only

TJAP1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68495-1-PBS

产品说明书

CloneNo. 1H1F10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

1H1F10, PILT, Tight junction-associated protein 1, TJAP 1, TJP4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68495-1-PBS targets TJAP1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11807

Product name: Recombinant human TJAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-351 aa of BC064401

Sequence: MTSAAPAKKPYRKAPPEHRELRLEIPGSRLEQEEPLTDAERMKLLQEENEELRRRLASATRRTEALERELEIGQDCLELELGQSREELDKFKDKFRRLQNSYTASQRTNQELEDKLHTLIKKAEMDRKTLDWEIVELTNKLLDAKNTINKLEELNERYRLDCNLAVQLLKCNKSHFRNHKFADLPCELQDMVRKHLHSGQEAASPGPAPSLAPGAVVPTSVIARVLEKPESLLLNSAQSGSAGRPLAEDVFVHVDMSEGVPGDPASPPAPGSPTPQPNGECHSLGTARGSPEEELPLPAFEKLNPYPTPSPPHPLYPGRRVIEFSEDKVRIPRNSPLPNCTYATRQAISLS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tight junction associated protein 1 (peripheral)
别名 1H1F10, PILT, Tight junction-associated protein 1, TJAP 1, TJP4
计算分子量 547 aa, 61 kDa
观测分子量 86-95 kDa
GenBank蛋白编号BC064401
基因名称 TJAP1
Gene ID (NCBI) 93643
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ5JTD0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TJAP1, also named as PILT and TJP4, is colocalized at the tight junctions. TJAP1 has some forms with MW 60 kDa (native) and 86-90 kDa (modified).

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